Journal:

Article Title: Upregulation of CD40 Expression on Endothelial Cells Infected with Human Cytomegalovirus

doi: 10.1128/JVI.76.24.12803-12812.2002

Figure Lengend Snippet: Immunoblot analysis of CD40 in whole-cell lysates. Cell monolayers were infected with HCMV TB40E at an MOI of 1 (inf.) or mock infected (noninfected [n.i.]). Cell lysates were prepared at the indicated times and analyzed by immunoblotting. (A) CD40 protein levels were detected with a polyclonal rabbit serum directed against human CD40. (B) Blots from panel A were stripped and reprobed with a rabbit serum directed against calreticulin. (C) Signal intensities from panels A and B were densitometrically quantified; data represent the ratio of CD40 to calreticulin (y axis) versus time p.i. (x axis). (D) Protein lysates from infected or noninfected cells were treated with N-glycosidase F (+) or left untreated (−) prior to immunoblot analysis with anti-CD40 antibodies. Molecular masses are given in kilodaltons.

Article Snippet: The following monoclonal antibodies or polyclonal sera were used: mouse IgG1-fluorescein isothiocyanate (FITC)-conjugated anti-human CD54 (Calbiochem, La Jolla, Calif.), anti-human major histocompatibility complex class I (MHC-I) (BD PharMingen, San Diego, Calif.), mouse IgG1-FITC anti-human CD40 (Dianova, Hamburg, Germany), mouse IgG1-FITC anti-human CD62E (Calbiochem), antibodies G28.5 and Ro1 (anti-human CD40) (gifts from H. Engelmann, Institute for Immunology, University of Munich, Munich, Germany) ( 39 ), polyclonal rabbit anti-CD40 serum (Santa Cruz Biotechnology), rabbit antiserum against human calreticulin (Dianova), biotin-conjugated rabbit anti-mouse antibodies, and biotinylated swine anti-rabbit antibodies (Dako, Hamburg, Germany).

Techniques: Western Blot, Infection

Journal: Oncology Letters

Article Title: Enhanced SOX2 expression in retinoblastoma tissues and peripheral blood is associated with the clinicopathological characteristics of the disease

doi: 10.3892/ol.2015.2857

Figure Lengend Snippet: Primers for quantitative reverse transcription polymerase chain reaction.

Article Snippet: Subsequent to blocking non-specific binding with horse serum, the polyclonal rabbit anti-human SOX2 antibody (dilution, 1:200; cat. no. ab171380) was added, followed by a 30-min incubation with biotin-labeled polyclonal goat anti-mouse IgG (dilution, 1:3,000; cat. no ab6789) and polyclonal goat anti-rabit IgG (dilution, 1:2,000; cat. no. ab6721) secondary antibodies (Abcam, Cambridge, MA, USA) at 37°C.

Techniques: Sequencing

Journal: Oncology Letters

Article Title: Enhanced SOX2 expression in retinoblastoma tissues and peripheral blood is associated with the clinicopathological characteristics of the disease

doi: 10.3892/ol.2015.2857

Figure Lengend Snippet: Immunohistochemical analysis revealing the expression of sex-determining region Y box 2 (SOX2) protein in retinoblastoma tissues (magnification, ×10). (A) Strong positive expression of SOX2. (B) Positive expression of SOX2. (C) Weak positive expression of SOX2 in the cytoplasm. (D) Positive expression of SOX2 in the nucleus.

Article Snippet: Subsequent to blocking non-specific binding with horse serum, the polyclonal rabbit anti-human SOX2 antibody (dilution, 1:200; cat. no. ab171380) was added, followed by a 30-min incubation with biotin-labeled polyclonal goat anti-mouse IgG (dilution, 1:3,000; cat. no ab6789) and polyclonal goat anti-rabit IgG (dilution, 1:2,000; cat. no. ab6721) secondary antibodies (Abcam, Cambridge, MA, USA) at 37°C.

Techniques: Immunohistochemical staining, Expressing

Journal: Oncology Letters

Article Title: Enhanced SOX2 expression in retinoblastoma tissues and peripheral blood is associated with the clinicopathological characteristics of the disease

doi: 10.3892/ol.2015.2857

Figure Lengend Snippet: Correlation of sex-determining region Y box 2 (SOX2) expression and clinicopathological features of retinoblastoma.

Article Snippet: Subsequent to blocking non-specific binding with horse serum, the polyclonal rabbit anti-human SOX2 antibody (dilution, 1:200; cat. no. ab171380) was added, followed by a 30-min incubation with biotin-labeled polyclonal goat anti-mouse IgG (dilution, 1:3,000; cat. no ab6789) and polyclonal goat anti-rabit IgG (dilution, 1:2,000; cat. no. ab6721) secondary antibodies (Abcam, Cambridge, MA, USA) at 37°C.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Enhanced SOX2 expression in retinoblastoma tissues and peripheral blood is associated with the clinicopathological characteristics of the disease

doi: 10.3892/ol.2015.2857

Figure Lengend Snippet: Sex-determining region Y box 2 (SOX2) protein expression in retinoblastoma (Rb) tissues, as detected by western blot analysis. The quantification of western blots were plotted into histograms. (A) SOX2 protein expression in Rb and control tissues. * P<0.05, compared with the control group. (B) SOX2 protein expression in the poorly and well-differentiated groups. * P<0.05, compared with the poorly differentiated group. (C) SOX2 protein expression in Rb tissues at stages N0, N1, N2 and N3. * P<0.05, compared with the N0 group. All data are presented as the mean ± standard error of the mean.

Article Snippet: Subsequent to blocking non-specific binding with horse serum, the polyclonal rabbit anti-human SOX2 antibody (dilution, 1:200; cat. no. ab171380) was added, followed by a 30-min incubation with biotin-labeled polyclonal goat anti-mouse IgG (dilution, 1:3,000; cat. no ab6789) and polyclonal goat anti-rabit IgG (dilution, 1:2,000; cat. no. ab6721) secondary antibodies (Abcam, Cambridge, MA, USA) at 37°C.

Techniques: Expressing, Western Blot

Journal: Oncology Letters

Article Title: Enhanced SOX2 expression in retinoblastoma tissues and peripheral blood is associated with the clinicopathological characteristics of the disease

doi: 10.3892/ol.2015.2857

Figure Lengend Snippet: Sex-determining region Y box 2 (SOX2) gene expression in the peripheral blood of children with retinoblastoma (Rb). The quantification of the quantitative reverse transcription polymerase chain reactions were plotted into histograms. (A) Relative expression of SOX2 mRNA in the peripheral blood of control individuals and Rb patients. * P<0.05, compared with the control group. (B) Expression of SOX2 mRNA in the peripheral blood of the poorly and well-differentiated groups. * P<0.05, compared with the poorly differentiated group. All data are presented as the mean ± standard error of the mean.

Article Snippet: Subsequent to blocking non-specific binding with horse serum, the polyclonal rabbit anti-human SOX2 antibody (dilution, 1:200; cat. no. ab171380) was added, followed by a 30-min incubation with biotin-labeled polyclonal goat anti-mouse IgG (dilution, 1:3,000; cat. no ab6789) and polyclonal goat anti-rabit IgG (dilution, 1:2,000; cat. no. ab6721) secondary antibodies (Abcam, Cambridge, MA, USA) at 37°C.

Techniques: Expressing